| Continentalcup1: | Folded |
| Continentalcup2: | New Clubs in League |
| Nextseason: | 1901–02 |
The 1900–01 season was the 13th season of The Football League.
Beginning in the 1894–95 season, clubs finishing level on points were separated according to goal average (goals scored divided by goals conceded). In case one or more teams had the same goal difference, this system favoured those teams who had scored fewer goals. The goal average system was eventually scrapped beginning with the 1976–77 season.
During the first six seasons of the league, (up to the 1893–94 season), re-election process concerned the clubs which finished in the bottom four of the league. From the 1894–95 season and until the 1920–21 season the re-election process was required of the clubs which finished in the bottom three of the league.
| Competition: | First Division |
| Season: | 1900–01 |
| Winners: | Liverpool 1st English title |
| Relegated: | Preston North End West Bromwich Albion |
| League Topscorer: | Steve Bloomer (23 goals) |
| Biggest Home Win: | (1 December 1900) |
| Biggest Away Win: | (20 October 1900) |
| Highest Scoring: | (22 September 1900) (8 December 1900) (12 January 1901) |
| Matches: | 306 |
| Total Goals: | 855 |
| Longest Wins: | 5 matches Nottingham Forest |
| Longest Unbeaten: | 12 matches Liverpool Nottingham Forest |
| Longest Losses: | 6 matches Preston North End |
| Highest Attendance: | 50,000 Everton 1–1 Liverpool (22 September 1900) |
| Lowest Attendance: | 1,050 West Bromwich Albion 0–2 Sheffield United (30 April 1901) |
| Average Attendance: | 11,151 |
| Prevseason: | 1899–1900 |
| Nextseason: | 1901–02 |
| Competition: | Second Division |
| Season: | 1900–01 |
| Winners: | Grimsby Town |
| Promoted: | Grimsby Town Small Heath |
| Relegated: | Walsall |
| League Topscorer: | Andrew Swann (18 goals)[1] |
| Biggest Home Win: | (2 March 1901) |
| Biggest Away Win: | (24 September 1900) |
| Highest Scoring: | (9 March 1901) |
| Matches: | 306 |
| Total Goals: | 819 |
| Longest Wins: | 5 matches Burnley Grimsby Town Middlesbrough |
| Longest Unbeaten: | 17 matches Grimsby Town |
| Longest Losses: | 5 matches Barnsley Chesterfield Gainsborough Trinity Newton Heath |
| Prevseason: | 1899–1900 |
| Nextseason: | 1901–02 |