The 1900–01 Northern Football League season was the twelfth in the history of the Northern Football League, a football competition in Northern England. The league reverted to a single division after having two divisions for the previous three seasons.
| Competition: | Northern League |
| Season: | 1900–01 |
| Winners: | Bishop Auckland |
| Matches: | 110 |
| Total Goals: | 386 |
| Prevseason: | 1899–1900 |
| Nextseason: | 1901–02 |
The league featured eight clubs which competed in the last season, along with three new clubs, promoted from last seasons's Division Two: