| Score: | 7–5, 6–2 |
| Draw: | 8 + 1 |
| Seeds: | – |
See main article: article and 1889 U.S. National Championships (tennis). Bertha Townsend defended her tennis singles title by defeating Lida Voorhees 7–5, 6–2 in the Challenge Round of the 1889 U.S. Women's National Singles Championship. Voorhuis had won the right to challenge Townsend by defeating Helen Day Harris 6–5, 2–6, 6–3 in the final of the All Comers' competition. The event was held at the Philadelphia Cricket Club, Pennsylvania.[1]