16S rRNA pseudouridine516 synthase explained

16S rRNA pseudouridine^516 synthase
Ec Number:	5.4.99.19

16S rRNA pseudouridine⁵¹⁶ synthase (16S RNA pseudouridine⁵¹⁶ synthase, 16S PsiI⁵¹⁶ synthase, 16S RNA Psi⁵¹⁶ synthase, RNA pseudouridine synthase RsuA, RsuA, 16S RNA pseudouridine 516 synthase) is an enzyme with systematic name 16S rRNA-uridine⁵¹⁶ uracil mutase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

16S rRNA uridine⁵¹⁶

\rightleftharpoons 16S rRNA pseudouridine⁵¹⁶

The enzyme is specific for uridine⁵¹⁶ in 16S rRNA.

Notes and References

1. Wrzesinski J, Bakin A, Nurse K, Lane BG, Ofengand J . Purification, cloning, and properties of the 16S RNA pseudouridine 516 synthase from Escherichia coli . Biochemistry . 34 . 27 . 8904–13 . July 1995 . 7612632 . 10.1021/bi00027a043 .
2. Conrad J, Niu L, Rudd K, Lane BG, Ofengand J . 16S ribosomal RNA pseudouridine synthase RsuA of Escherichia coli: deletion, mutation of the conserved Asp102 residue, and sequence comparison among all other pseudouridine synthases . RNA . 5 . 6 . 751–63 . June 1999 . 10376875 . 1369802 . 10.1017/s1355838299990167 .
3. Sivaraman J, Sauvé V, Larocque R, Stura EA, Schrag JD, Cygler M, Matte A . Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP . Nature Structural Biology . 9 . 5 . 353–8 . May 2002 . 11953756 . 10.1038/nsb788 . 22037795 .