16S rRNA (cytosine967-C5)-methyltransferase explained

16S rRNA (cytosine^967-C^5)-methyltransferase
Ec Number:	2.1.1.176

16S rRNA (cytosine⁹⁶⁷-C⁵)-methyltransferase (rsmB (gene), fmu (gene), 16S rRNA m5C967 methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:16S rRNA (cytosine⁹⁶⁷-C⁵)-methyltransferase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

\rightleftharpoons S-adenosyl-L-homocysteine + 5-methylcytosine⁹⁶⁷ in 16S rRNA

The enzyme specifically methylates cytosine⁹⁶⁷ at C⁵ in 16S rRNA.

Notes and References

1. Tscherne JS, Nurse K, Popienick P, Michel H, Sochacki M, Ofengand J . Purification, cloning, and characterization of the 16S RNA m5C967 methyltransferase from Escherichia coli . Biochemistry . 38 . 6 . 1884–92 . February 1999 . 10026269 . 10.1021/bi981880l .
2. Gu XR, Gustafsson C, Ku J, Yu M, Santi DV . Identification of the 16S rRNA m5C967 methyltransferase from Escherichia coli . Biochemistry . 38 . 13 . 4053–7 . March 1999 . 10194318 . 10.1021/bi982364y .
3. Foster PG, Nunes CR, Greene P, Moustakas D, Stroud RM . The first structure of an RNA m5C methyltransferase, Fmu, provides insight into catalytic mechanism and specific binding of RNA substrate . Structure . 11 . 12 . 1609–20 . December 2003 . 14656444 . 10.1016/j.str.2003.10.014 . free .