| District | Senator | Party | Constituency | Began serving |
|---|
| 1st | | John H. Hassinger | Republican | Washington, Smyth, and city of Bristol | 1920 |
| 2nd | | Charles S. Pendleton | Republican | Scott, Lee, and Wise | 1920 |
| 3rd | | Robert O. Crockett | Republican | Buchanan, Dickenson, Russell, and Tazewell | 1920 |
| 4th | | William L. Andrews | Democratic | Roanoke, Montgomery, and cities of Roanoke and Radford | 1915 |
| 5th | | E. Lee Trinkle | Democratic | Giles, Bland, Pulaski, and Wythe | 1916 |
| 6th | | John M. Parsons | Republican | Carroll, Grayson, and Patrick | 1920
(previously served 1908-1912) |
| 7th | | George W. Layman | Democratic | Craig, Botetourt, Allegheny, Bath, and city of Clifton Forge | 1920 |
| 8th | | John Paul, Jr. | Republican | Rockingham | 1920
(previously served 1912-1916) |
| 9th | | F. Percy Loth | Republican | Augusta, Highland, and city of Staunton | 1920 |
| 10th | | Harry F. Byrd | Democratic | Shenandoah, Frederick, and city of Winchester | 1916 |
| 11th | | J. Bradshaw Beverley | Democratic | Fauquier and Loudoun | 1920 |
| 12th | | Robert F. Leedy | Democratic | Clarke, Page, and Warren | 1919 |
| 13th | | C. O'Conor Goolrick | Democratic | Spotsylvania, Stafford, Louisa, and city of Fredericksburg | 1915 |
| 14th | | Walter T. Oliver | Democratic | Alexandria county, Prince William, Fairfax, and city of Alexandria | 1920 |
| 15th | | John J. Miller | Democratic | Culpeper, Madison, Rappahannock, and Orange | 1920 |
| 16th | | Thomas S. Hening | Democratic | Goochland, Powhatan, and Chesterfield | 1916 |
| 17th | | Nathaniel B. Early | Democratic | Albemarle, Greene, and city of Charlottesville | 1908 |
| 18th | | Samuel L. Ferguson | Democratic | Appomattox, Buckingham, Fluvanna, and Charlotte | 1919 |
| 19th | | J. Belmont Woodson | Democratic | Amherst and Nelson | 1916
(previously served 1906-1912) |
| 20th | | Robert A. Russell | Democratic | Campbell and city of Lynchburg | 1920 |
| 21st | | Marshall B. Booker | Democratic | Halifax | 1919 |
| 22nd | | A. Willis Robertson | Democratic | Bedford, Rockbridge, and city of Buena Vista | 1916 |
| 23rd | | William A. Garrett | Democratic | Pittsylvania, Henry, and city of Danville | 1901 |
| 24th | | George T. Rison | Democratic | Pittsylvania and city of Danville | 1904 |
| 25th | | William H. Jeffreys, Jr. | Democratic | Mecklenburg and Brunswick | 1916 |
| 26th | | Samuel G. Proffit | Democratic | Franklin and Floyd | 1920 |
| 27th | | William B. Cocke | Democratic | Greensville, Sussex, Surry, and Prince George | 1920 |
| 28th | | Louis S. Epes | Democratic | Nottoway, Amelia, Lunenburg, Prince Edward, and Cumberland | 1920 |
| 29th | | Patrick H. Drewry | Democratic | Dinwiddie and city of Petersburg | 1912 |
| 30th | | Junius E. West | Democratic | Isle of Wight, Southampton, and Nansemond | 1912 |
| 31st | | Joseph T. Deal | Democratic | Norfolk city | 1920 |
| 32nd | | Charles U. Gravatt | Democratic | Caroline, Hanover, and King William | 1908 |
| 33rd | | William C. Corbitt | Democratic | Norfolk county and city of Portsmouth | 1915 |
| 34th | | Thomas J. Downing | Democratic | King George, Richmond, Westmoreland, Lancaster, and Northumberland | 1920 |
| 35th | | Julien Gunn | Democratic | Henrico, New Kent, Charles City, James City, and city of Williamsburg | 1916 |
| 36th | | Saxon W. Holt | Democratic | Elizabeth City, York, Warwick, and city of Newport News | 1904 |
| 37th | | G. Walter Mapp | Democratic | Accomac, Northampton, and Princess Anne | 1912 |
| 38th | | James E. Cannon | Democratic | Richmond city | 1914 |
| | Morgan R. Mills | Democratic | 1920 |
| 39th | | J. Douglas Mitchell | Democratic | King and Queen, Middlesex, Essex, Gloucester, and Mathews | 1918 | |
