| District | Senator | Party | Constituency | Began serving |
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| 1st | | John P. Buchanan | Democratic | Washington, Smyth, and city of Bristol | 1916 |
| 2nd | | John M. Goodloe | Republican | Scott, Lee, and Wise | 1916 |
| 3rd | | J. Powell Royall | Republican | Buchanan, Dickenson, Russell, and Tazewell | 1912 |
| 4th | | William L. Andrews | Democratic | Roanoke, Montgomery, and cities of Roanoke and Radford | 1915 |
| 5th | | E. Lee Trinkle | Democratic | Giles, Bland, Pulaski, and Wythe | 1916 |
| 6th | | M. Price Webb | Republican | Carroll, Grayson, and Patrick | 1916 |
| 7th | | William A. Rinehart | Democratic | Craig, Botetourt, Allegheny, Bath, and city of Clifton Forge | 1912 |
| 8th | | George N. Conrad | Democratic | Rockingham | 1916 |
| 9th | | Cornelius T. Jordan | Independent | Augusta, Highland, and city of Staunton | 1916 |
| 10th | | Harry F. Byrd | Democratic | Shenandoah, Frederick, and city of Winchester | 1916 |
| 11th | | Lucien Keith | Democratic | Fauquier and Loudoun | 1918 |
| 12th | | Henry H. Downing | Democratic | Clarke, Page, and Warren | 1916 |
| 13th | | C. O'Conor Goolrick | Democratic | Spotsylvania, Stafford, Louisa, and city of Fredericksburg | 1915 |
| 14th | | R. Ewell Thornton | Democratic | Alexandria county, Prince William, Fairfax, and city of Alexandria | 1908 |
| 15th | | Clyde T. Bowers | Democratic | Culpeper, Madison, Rappahannock, and Orange | 1912 |
| 16th | | Thomas S. Hening | Democratic | Goochland, Powhatan, and Chesterfield | 1916 |
| 17th | | Nathaniel B. Early | Democratic | Albemarle, Greene, and city of Charlottesville | 1908 |
| 18th | | Sands Gayle | Democratic | Appomattox, Buckingham, Fluvanna, and Charlotte | 1910 |
| 19th | | Aubrey E. Strode | Democratic | Amherst and Nelson | 1916 (previously served 1906-1912) |
| 20th | | Walter E. Addison | Democratic | Campbell and city of Lynchburg | 1916 |
| 21st | | James T. Lacy | Democratic | Halifax | 1916 |
| 22nd | | A. Willis Robertson | Democratic | Bedford, Rockbridge, and city of Buena Vista | 1916 |
| 23rd | | William A. Garrett | Democratic | Pittsylvania, Henry, and city of Danville | 1901 |
| 24th | | George T. Rison | Democratic | Pittsylvania and city of Danville | 1904 |
| 25th | | William H. Jeffreys, Jr. | Democratic | Mecklenburg and Brunswick | 1916 |
| 26th | | Beverly A. Davis | Republican | Franklin and Floyd | 1916 |
| 27th | | Sidney B. Barham, Jr. | Democratic | Greensville, Sussex, Surry, and Prince George | 1916 |
| 28th | | George E. Allen | Democratic | Nottoway, Amelia, Lunenburg, Prince Edward, and Cumberland | 1916 |
| 29th | | Patrick H. Drewry | Democratic | Dinwiddie and city of Petersburg | 1912 |
| 30th | | Junius E. West | Democratic | Isle of Wight, Southampton, and Nansemond | 1912 |
| 31st | | Earl C. Mathews | Democratic | Norfolk city | 1916 |
| 32nd | | Charles U. Gravatt | Democratic | Caroline, Hanover, and King William | 1908 |
| 33rd | | William C. Corbitt | Democratic | Norfolk county and city of Portsmouth | 1915 |
| 34th | | C. Harding Walker | Democratic | King George, Richmond, Westmoreland, Lancaster, and Northumberland | 1899 |
| 35th | | Julien Gunn | Democratic | Henrico, New Kent, Charles City, James City, and city of Williamsburg | 1916 |
| 36th | | Saxon W. Holt | Democratic | Elizabeth City, York, Warwick, and city of Newport News | 1904 |
| 37th | | G. Walter Mapp | Democratic | Accomac, Northampton, and Princess Anne | 1912 |
| 38th | | James E. Cannon | Democratic | Richmond city | 1914 |
| | Louis O. Wendenburg | Democratic | 1912 |
| 39th | | J. Douglass Mitchell | Democratic | King and Queen, Middlesex, Essex, Gloucester, and Mathews | 1918 | |