| District | Senator | Party | Constituency | Began serving |
|---|
| 1st | | David C. Cummings, Jr. | Democratic | Washington, Smyth, and city of Bristol | 1912 |
| 2nd | | John H. Catron | Republican | Scott, Lee, and Wise | 1912 |
| 3rd | | J. Powell Royall | Republican | Buchanan, Dickenson, Russell, and Tazewell | 1912 |
| 4th | | John M. Hart | Democratic | Roanoke, Montgomery, and cities of Roanoke and Radford | 1908 |
| 5th | | Alexander G. Crockett | Democratic | Giles, Bland, Pulaski, and Wythe | 1912 |
| 6th | | Edmund Parr | Republican | Carroll, Grayson, and Patrick | 1912
(previously served 1895-1899) |
| 7th | | William A. Rinehart | Democratic | Craig, Botetourt, Allegheny, Bath, and city of Clifton Forge | 1912 |
| 8th | | John Paul, Jr. | Republican | Rockingham | 1912 |
| 9th | | Edward Echols | Democratic | Augusta, Highland, and city of Staunton | 1906
(previously served 1889-1897) |
| 10th | | Frank S. Tavenner | Democratic | Shenandoah, Frederick, and city of Winchester | 1912
(previously served 1904-1908) |
| 11th | | G. Latham Fletcher | Democratic | Fauquier and Loudoun | 1908 |
| 12th | | R. S. Blackburn Smith | Democratic | Clarke, Page, and Warren | 1912 |
| 13th | | Richard C. L. Moncure | Democratic | Spotsylvania, Stafford, Louisa, and city of Fredericksburg | 1912 |
| 14th | | R. Ewell Thornton | Democratic | Alexandria county, Prince William, Fairfax, and city of Alexandria | 1908 |
| 15th | | Clyde T. Bowers | Democratic | Culpeper, Madison, Rappahannock, and Orange | 1912 |
| 16th | | John B. Watkins | Democratic | Goochland, Powhatan, and Chesterfield | 1908 |
| 17th | | Nathaniel B. Early | Democratic | Albemarle, Greene, and city of Charlottesville | 1908 |
| 18th | | Sands Gayle | Democratic | Appomattox, Buckingham, Fluvanna, and Charlotte | 1910 |
| 19th | | Bland Massie | Democratic | Amherst and Nelson | 1912
(previously served 1897-1906) |
| 20th | | Howell C. Featherston | Democratic | Campbell and city of Lynchburg | 1912 |
| 21st | | Henry A. Edmondson | Democratic | Halifax | 1908 |
| 22nd | | J. Randolph Tucker | Democratic | Bedford, Rockbridge, and city of Buena Vista | 1908 |
| 23rd | | William A. Garrett | Democratic | Pittsylvania, Henry, and city of Danville | 1901 |
| 24th | | George T. Rison | Democratic | Pittsylvania and city of Danville | 1904 |
| 25th | | William D. Blanks | Democratic | Mecklenburg and Brunswick | 1912 |
| 26th | | Valentine M. Sowder | Republican | Franklin and Floyd | 1912 |
| 27th | | Alexander R. Hobbs | Democratic | Greensville, Sussex, Surry, and Prince George | 1901 |
| 28th | | Robert K. Brock | Democratic | Nottoway, Amelia, Lunenburg, Prince Edward, and Cumberland | 1912 |
| 29th | | Patrick H. Drewry | Democratic | Dinwiddie and city of Petersburg | 1912 |
| 30th | | Junius E. West | Democratic | Isle of Wight, Southampton, and Nansemond | 1912 |
| 31st | | John A. Lesner | Democratic | Norfolk city | 1908 |
| 32nd | | Charles U. Gravatt | Democratic | Caroline, Hanover, and King William | 1908 |
| 33rd | | Samuel T. Montague | Democratic | Norfolk county and city of Portsmouth | 1912 |
| 34th | | C. Harding Walker | Democratic | King George, Richmond, Westmoreland, Lancaster, and Northumberland | 1899 |
| 35th | | Louis O. Wendenburg | Democratic | Henrico, New Kent, Charles City, James City, and city of Williamsburg | 1912 |
| 36th | | Saxon W. Holt | Democratic | Elizabeth City, York, Warwick, and city of Newport News | 1904 |
| 37th | | G. Walter Mapp | Democratic | Accomac, Northampton, and Princess Anne | 1912 |
| 38th | | Elben C. Folkes | Democratic | Richmond city | 1908 |
| | Arthur C. Harman | Democratic | 1904 |
| 39th | | John R. Saunders | Democratic | King and Queen, Middlesex, Essex, Gloucester, and Mathews | 1908 | |
