1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) explained

1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)
Ec Number:	1.1.1.292

In enzymology, a 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) is an enzyme that catalyzes the chemical reaction

1,5-anhydro-D-mannitol + NADP

\rightleftharpoons 1,5-anhydro-D-fructose + NADPH + H

Thus, the two substrates of this enzyme are 1,5-anhydro-D-mannitol and NADP, whereas its 3 products are 1,5-anhydro-D-fructose, NADPH, and H.

This enzyme belongs to the common family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD or NADP as acceptor. The systematic name of this enzyme class is 1,5-anhydro-D-mannitol:NADP oxidoreductase. Other names in common use include 1,5-anhydro-D-fructose reductase (ambiguous), and AFR.

References

• Kuhn A, Yu S, Giffhorn F . 2006 . Catabolism of 1,5-anhydro-D-fructose in Sinorhizobium morelense S-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro-D-fructose reductase and its application in sugar analysis and rare sugar synthesis . Appl. Environ. Microbiol. . 72 . 1248 - 57 . 16461673 . 10.1128/AEM.72.2.1248-1257.2006 . 2 . 1392929 .
• Dambe TR, Kuhn AM, Brossette T, Giffhorn F, Scheidig AJ . 2006 . Crystal structure of NADP(H)-dependent 1,5-anhydro-D-fructose reductase from Sinorhizobium morelense at 2.2 Å resolution: construction of an NADH-accepting mutant and its application in rare sugar synthesis . Biochemistry . 45 . 10030 - 42 . 16906761 . 10.1021/bi052589q . 33 .