1,4-lactonase explained
| 1,4-lactonase |
| Ec Number: | 3.1.1.25 |
| Cas Number: | 37278-38-9 |
| Go Code: | 0050490 |
The enzyme 1,4-lactonase (EC 3.1.1.25) catalyzes the generic reaction
a 1,4-lactone + H2O
a 4-hydroxyacid
This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name is 1,4-lactone hydroxyacylhydrolase. It is also called γ-lactonase. It participates in galactose metabolism and ascorbate and aldarate metabolism. It employs one cofactor, Ca2+.
Structural studies
As of late 2007, three structures have been solved for this class of enzymes, with PDB accession codes,, and .
Applications
In a study by Chen et al. a 1,4-lactonase was expressed in E. coli and used as a highly efficient biocatalyst for asymmetric synthesis of chiral compounds.[1]
References
- Fishbein WN, Bessman SP . 1966 . Purification and properties of an enzyme in human blood and rat liver microsomes catalyzing the formation and hydrolysis of γ-lactones. I. Tissue localization, stoichiometry, specificity, distinction from esterase . J. Biol. Chem. . 241 . 4835 - 41 . 4958984 . 21 . 10.1016/S0021-9258(18)99641-9 . free .
- Fishbein WN, Bessman SP . 1966 . Purification and properties of an enzyme in human blood and rat liver microsomes catalyzing the formation and hydrolysis of γ-lactones. II. Metal ion effects, kinetics, and equilibra . J. Biol. Chem. . 241 . 4842 - 7 . 4958985 . 21 . 10.1016/S0021-9258(18)99642-0 . free .
Notes and References
- Chen. Bing. Fan. Li-Qiang. Xu. Jian-He. Zhao. Jian. Zhang. Xian. Ouyang. Li-Ming. October 2010. Biocatalytic properties of a recombinant Fusarium proliferatum lactonase with significantly enhanced production by optimal expression in Escherichia coli. Applied Biochemistry and Biotechnology. 162. 3. 744–756. 10.1007/s12010-009-8819-1. 1559-0291. 19876606.
