(butirosin acyl-carrier protein)—L-glutamate ligase explained

(butirosin acyl-carrier protein)—L-glutamate ligase
Ec Number:	6.2.1.39

(Butirosin acyl-carrier protein)—L-glutamate ligase ([BtrI acyl-carrier protein]—L-glutamate ligase, BtrJ) is an enzyme with systematic name (BtrI acyl-carrier protein):L-glutamate ligase (ADP-forming).^[1] This enzyme catalyses the following chemical reaction

(1) ATP + L-glutamate + BtrI acyl-carrier protein

\rightleftharpoons ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]

(2) ATP + L-glutamate + 4-amino butanoyl-[BtrI acyl-carrier protein]

\rightleftharpoons ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]

This enzyme catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family.

Notes and References

1. Li Y, Llewellyn NM, Giri R, Huang F, Spencer JB . Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway . Chemistry & Biology . 12 . 6 . 665–75 . June 2005 . 15975512 . 10.1016/j.chembiol.2005.04.010 . free .