(R)-amidase explained

(R)-amidase
Ec Number:	3.5.1.100

(R)-amidase (R-stereospecific amidase, R-amidase) is an enzyme with systematic name (R)-piperazine-2-carboxamide amidohydrolase.^[1] This enzyme catalyses the following chemical reaction

(1) (R)-piperazine-2-carboxamide + H₂O

\rightleftharpoons (R)-piperazine-2-carboxylic acid + NH₃

(2) beta-alaninamide + H₂O

\rightleftharpoons beta-alanine + NH₃

This enzyme also hydrolyses (R)-piperidine-3-carboxamide to (R)-piperidine-3-carboxylic acid and NH3.

Notes and References

1. Komeda H, Harada H, Washika S, Sakamoto T, Ueda M, Asano Y . A novel R-stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine-2-tert-butylcarboxamide . European Journal of Biochemistry . 271 . 8 . 1580–90 . April 2004 . 15066183 . 10.1111/j.1432-1033.2004.04069.x . 10.1.1.547.2141 .