(KDO)3-lipid IVA (2-4) 3-deoxy-D-manno-octulosonic acid transferase explained

(KDO)3-lipid IVA (2-4) 3-deoxy-D-manno-octulosonic acid transferase
Ec Number:	2.4.99.15

(KDO)3-lipid IVA (2-4) 3-deoxy-D-manno-octulosonic acid transferase (KDO transferase, waaA (gene), kdtA (gene), 3-deoxy-D-manno-oct-2-ulosonic acid transferase, 3-deoxy-manno-octulosonic acid transferase) is an enzyme with systematic name CMP-3-deoxy-D-manno-oct-2-ulosonate:(KDO)3-lipid IVA 3-deoxy-D-manno-oct-2-ulosonate transferase ((2->4) glycosidic bond-forming).^{[1] [2]} This enzyme catalyses the following chemical reaction

alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + CMP-alpha-Kdo

\rightleftharpoons alpha-Kdo-(2->8)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + CMP

The enzyme from Chlamydia psittaci transfers four KDO residues to lipid A, forming a branched tetrasaccharide.

Notes and References

1. Brabetz W, Lindner B, Brade H . Comparative analyses of secondary gene products of 3-deoxy-D-manno-oct-2-ulosonic acid transferases from Chlamydiaceae in Escherichia coli K-12 . European Journal of Biochemistry . 267 . 17 . 5458–65 . September 2000 . 10951204 . 10.1046/j.1432-1327.2000.01619.x . free .
2. Holst O, Bock K, Brade L, Brade H . The structures of oligosaccharide bisphosphates isolated from the lipopolysaccharide of a recombinant Escherichia coli strain expressing the gene gseA [3-deoxy-D-manno-octulopyranosonic acid (Kdo) transferase] of Chlamydia psittaci 6BC . European Journal of Biochemistry . 229 . 1 . 194–200 . April 1995 . 7744029 . 10.1111/j.1432-1033.1995.tb20455.x .