(KDO)2-lipid IVA (2-8) 3-deoxy-D-manno-octulosonic acid transferase explained

(KDO)2-lipid IVA (2-8) 3-deoxy-D-manno-octulosonic acid transferase
Ec Number:	2.4.99.14

(KDO)2-lipid IVA (2-8) 3-deoxy-D-manno-octulosonic acid transferase (KDO transferase, waaA (gene), kdtA (gene), 3-deoxy-D-manno-oct-2-ulosonic acid transferase, 3-deoxy-manno-octulosonic acid transferase) is an enzyme with systematic name CMP-3-deoxy-D-manno-oct-2-ulosonate:(KDO)2-lipid IVA 3-deoxy-D-manno-oct-2-ulosonate transferase ((2->8) glycosidic bond-forming).^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + CMP-alpha-Kdo

\rightleftharpoons alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + CMP

The enzymes from Chlamydia transfer three or more 3-deoxy-D-manno-oct-2-ulosonate residues and generate genus-specific epitopes.

Notes and References

1. Löbau S, Mamat U, Brabetz W, Brade H . Molecular cloning, sequence analysis, and functional characterization of the lipopolysaccharide biosynthetic gene kdtA encoding 3-deoxy-alpha-D-manno-octulosonic acid transferase of Chlamydia pneumoniae strain TW-183 . Molecular Microbiology . 18 . 3 . 391–9 . November 1995 . 8748024 . 10.1111/j.1365-2958.1995.mmi_18030391.x .
2. Mamat U, Baumann M, Schmidt G, Brade H . The genus-specific lipopolysaccharide epitope of Chlamydia is assembled in C. psittaci and C. trachomatis by glycosyltransferases of low homology . Molecular Microbiology . 10 . 5 . 935–41 . December 1993 . 7523826 . 10.1111/j.1365-2958.1993.tb00965.x .
3. Belunis CJ, Mdluli KE, Raetz CR, Nano FE . A novel 3-deoxy-D-manno-octulosonic acid transferase from Chlamydia trachomatis required for expression of the genus-specific epitope . The Journal of Biological Chemistry . 267 . 26 . 18702–7 . September 1992 . 1382060 .