Κ-Carrageenase Explained

κ-Carrageenase
Ec Number:	3.2.1.83
Cas Number:	37288-59-8

κ-Carrageenase (κ-carrageenan 4-β-D-glycanohydrolase) is an enzyme with systematic name κ-carrageenan 4-β-D-glycanohydrolase (configuration-retaining).^{[1] [2] [3] [4] [5]} It catalyses the endohydrolysis of (1→4)-β-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in κ-carrageenans

The main products of hydrolysis are neocarrabiose-sulfate and neocarratetraose-sulfate.

Notes and References

1. Weigl J, Yaphe W . The enzymic hydrolysis of carrageenan by Pseudomonas carrageenovora: purification of a κ-carrageenase . Canadian Journal of Microbiology . 12 . 5 . 939–47 . October 1966 . 5972647 . 10.1139/m66-127 .
2. Potin P, Sanseau A, Le Gall Y, Rochas C, Kloareg B . Purification and characterization of a new κ-carrageenase from a marine Cytophaga-like bacterium . European Journal of Biochemistry . 201 . 1 . 241–7 . October 1991 . 1915370 . 10.1111/j.1432-1033.1991.tb16280.x .
3. Potin P, Richard C, Barbeyron T, Henrissat B, Gey C, Petillot Y, Forest E, Dideberg O, Rochas C, Kloareg B . Processing and hydrolytic mechanism of the cgkA-encoded κ-carrageenase of Alteromonas carrageenovora . European Journal of Biochemistry . 228 . 3 . 971–5 . March 1995 . 7737202 . 10.1111/j.1432-1033.1995.tb20348.x .
4. Michel G, Barbeyron T, Flament D, Vernet T, Kloareg B, Dideberg O . Expression, purification, crystallization and preliminary x-ray analysis of the κ-carrageenase from Pseudoalteromonas carrageenovora . Acta Crystallographica Section D . 55 . Pt 4 . 918–20 . April 1999 . 10089334 . 10.1107/s0907444998018526 .
5. Michel G, Chantalat L, Duee E, Barbeyron T, Henrissat B, Kloareg B, Dideberg O . The κ-carrageenase of P. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of Clan-B glycoside hydrolases . Structure . 9 . 6 . 513–25 . June 2001 . 11435116 . 10.1016/s0969-2126(01)00612-8 .