Α,α-Trehalase explained

α,α-Trehalase
Ec Number:	3.2.1.28
Cas Number:	9025-52-9
Go Code:	0004555

An α,α-trehalase is an enzyme with systematic name α,α-trehalose glucohydrolase.^{[1] [2] [3] [4]} This enzyme catalyzes the chemical reaction

α,α-trehalose + H₂O

\rightleftharpoons 2 D-glucose

This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. It is also called trehalase, and it participates in starch and sucrose metabolism.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes and .

Notes and References

1. Trehalose und Hefe. II. Trehalasewirkung von Hefepräparaten . Myrbäck K, Örtenblad B . Biochem. Z. . 1937 . 291 . 61–69 .
2. Kalf GF, Rieder SV . The purification and properties of trehalase . The Journal of Biological Chemistry . 230 . 2 . 691–8 . February 1958 . 13525386 .
3. Hehre EJ, Sawai T, Brewer CF, Nakano M, Kanda T . Trehalase: stereocomplementary hydrolytic and glucosyl transfer reactions with α- and β-D-glucosyl fluoride . Biochemistry . 21 . 13 . 3090–7 . June 1982 . 7104311 . 10.1021/bi00256a009 .
4. Mori H, Lee JH, Okuyama M, Nishimoto M, Ohguchi M, Kim D, Kimura A, Chiba S . Catalytic reaction mechanism based on α-secondary deuterium isotope effects in hydrolysis of trehalose by European honeybee trehalase . Bioscience, Biotechnology, and Biochemistry . 73 . 11 . 2466–73 . November 2009 . 19897915 . 10.1271/bbb.90447 . 22774772 . free .